pGLO Transformation Lab

 

Results from our lab. The four plates above are the +LB/amp,+ LB/amp/ara,-LB/amp, and the -LB.
The +LB/amp was the antibotic, the + LB/amp/ara was the arabinose sugar, and the negative plates were the plasmids.

 



This lab that we did in class was about how we collected information about the +pGLO cells with E coli grown on the plates. The plates that I would expect to find bacteria like the original non-transformed E.coli colonies that were intially observed would be on the LB plate because you can hopefully see everything growing because it is cabale of doing it. The LB/amp/ara plates would genetically have transformed bacterial cells because it would have had to take the DNA from the Amp in order to survive. The -pGLO and + pGLO plates should be compared to dertermine if any genetic transformation has occured because it would tell you if the plasmid transformed them. The negative plates were the control plates.

Observations that we collected was how there was alot of bacterial colonies on the + LB/amp, and there was around 35 colonies. The bacteria is a fluroscent green color. The bacteria in this plate didn't glow. On the + LB/amp/ara there was a less amount of bacterial colonies, and there was around 25 colonies. The bacteria was also a fluroscent green color. The spots on this plate glowed because it got a signal from the environment. The - LB/amp didn't have any spots, it was just a smear which was a fluroscent green color. Nothing occured on the - LB plate because there was just condensation on the plate. The traits that was originally observed for E.coli that was not altered was the colony shape and color. Traits that were different after performing the transformation procedure was the colony shape because there was a less amount of dots on the two positve plates, and on the -LB/amp the shape was totally different because there wasn't any dots. If the genetically transformed cells were acquired the ability to live in the presence of the antibiotic ampicillin then the other genes on the plasmid would have no bacteria growing. The changes that occured were due to the procedure that was performed because the + LB/amp didn't glow due to that glowing will only happen in the presence of arabinose. With the plasmid, the genes that code for the enzyme that digest arabinose are replaced with the GFP. So, without arabinose the gene is no longer working so that is why it didn't glow. In conclusion, I learned alot of neat information by doing this lab, and the transformation about pGLO cells with Ecoli grown on the plates.

Movie GATTACA

The movie GATTACA that we watched in class was a fascinating movie about a guy who posed as someone else to pursue a life long dream of going to space. I think vincent tore his photo out of the family photo after winning the swimming race against his brother because I think he wanted his family to appreciate what he did; and to prove to his brother that he could strive in life and that he could do whatever he wanted even though his DNA claimed he had a heart condition. Anton didn't have the drive in life, like Vincent did. The relationship between Vincent and Anton wasn't very good because they didn't talk to one another in years. My favorite character in this movie was Vincent because he did extraordinary things to pose as Jerome to go into space without getting caught. I would have wanted to be Vincent because I think that it would be really fun to pose as someone else, but I would be very worried about getting caught. Three ways that society portrayed in this movie that usually reads a person's genetic profile is by hair, saliva, blood, and urine.

I think that Vincent's world could eventually happen in America because I think that we will be able to have the technology to do it. If I was Anton in this film I would have had a better ambition in life, and would have strived harder then what he did. I would have had more confidence in Vincent then what he did. I would have told him that I knew he could make his dream come true. I think that the doctor went along with Vincent's fraud because he wanted him to be able to pursue his dream because he knew that it was very important to him. If I was the doctor I would have let him go to, so I would have done the same thing.  In conclusion, this movie was very interesting and I learned alot about DNA, and all of the great technology that will probably be available in America one day to pose as someone else.

DNA Sequence Comparisons Analysis

For the results of Abby there was a 97% similarity. There was one base change because there was a change between the A and the T because it should have been a GAG and instead the results was a GTG. This process is called point mutation. I don't think that she has a disease because most of her DNA was the same.

For the results of Bob there was a 97% similarity. There was not enough protein produced once it reached the STOP signal. He has a disease. This process is called truncation mutation.

For the results of Carol there was a 58% similarity. In the frame, she was only missing the T. So, only one changed out of the sequence. She has a disease. This process is called Frame Shift mutation.

DNA Lab



This is what the formation started to look like in the tube.

This is what DNA looks like from the results of the lab.



      DNA Is totally amazing!!! Well to start with, DNA is a polymer. The monomer units of DNA are called nucleotides. The polymer is called a polynucleotide. Each nucleotide consists of a 5-carbon sugar, a nitrogen containing a base that is attached to the sugar, and a phosphate group. There are four different types of nucleotides that are found in DNA which are A, G, C, and T. A is for adenine, G is for guanine, C is for cytosine, and T is for thymine. In purine bases which are adenine and guanine there are nine atoms that make up the fused rings, and all of the ring atoms lie in the same plane.

The lab that we did in class was DNA Extraction from Wheat Germ. The wheat germ soup looked like a watery brown color to begin with. Then the appearance changed as my group added detergent and swirl into it. It turned to a lime green color with brown speks. At this step it is getting into the process of creating DNA. The appearance of the mixture after the alcohol was added, was that there was five different layers and now it has created DNA. At this step DNA has been created because of the formation it has went through. At the water-alcohol interface it didn't mix and it was staying separated. At the end of this lab the DNA looked like a white clump, like a spider web. In Conclusion, I learned about the formation of DNA by doing this neat lab.

From DNA to Proteins Vocabulary Words

Anticodon - A sequence of three nucleotides in transfer RNA that binds to the complementary triplet in messenger RNA to specify an amino acid during protein synthesis.

Codon - A sequence of three adjacent nucleotides, which encode for a specific amino acid during protein synthesis, or translation.

Exon -A sequence of a gene's DNA that transcribes into protein structures.

Genetic Code -The ordering of nucleotides in DNA molecules that carries the genetic information in   living cells.

Intron - A sequence of a eukaryotic gene's DNA that is not translated into a protein.

Messenger RNA -The template for protein synthesis; the form of RNA that carries information from DNA in the nucleus to the ribosome sites of protein synthesis in the cell.

Promoter - A region of DNA that facilitates the transcription of a particular gene.

Protein-coding Gene -Consists of a promoter that is followed by the coding sequence for the protein and then a terminator.

Ribonucleic Acid (RNA) -A nucleic acid molecule that is similar to DNA, but it contains ribose rather than deoxyribose.

RNA polymerase - An enzyme that produces RNA.

Ribosomal ribonucleic acid (rRNA) -Is the central component of the ribosome, the protein manufacturing machinery of all living cells.

Transcription -Or RNA synthesis. It is the process of creating an equivalent RNA copy of a sequence of DNA.

Translation -The process whereby genetic information coded in messenger RNA directs the formation of a specific protein at a ribosome in the cytoplasm.

Transfer RNA (tRNA) -A relatively small RNA that transfers a particular amino acid to a growing polypeptide chain at the ribosomal site of protein synthesis during translation.

In Sickness and in Health Genetic Counseling

The pedigree for Greg's Family 




This is an example of an autosomal dominant inheritance pattern.

 



The Pedigree for Olga's Family

        Many people wonder well could our kids develop the same diseases that run in our family? Greg and Olga were wondering the same thing. Well it's all genetics!!! For the Sickness and Health Genetic Counseling Greg and Olga went to the genetic's counselor because they were worried about having kids because they were concerned whether or not their kids will have myotonic dystrophy or factor VIII. Autosomal dominant disorders don't skip generations. So, Greg and his mother couldn't be factors of the gene that causes myotonic dystrophy. There is not a possibility that Greg's aunt or uncle could be homozygous for the myotonic dystrophy because they only got one copy. So, they are both heterozygous. There is not a possibility that Greg's cousin has inherited the myotonic dystrophy gene. Greg and Olga's children won't have myotonic dystrophy because Greg doesn't have the disease. The five hallmarks of autosomal recessive traits are females and males are equally likely to be affected, on average, the recurrence risk to the unborn sibling of an affected individual is one out of four, the trait is characteristically found in siblings, not parents of affected or the offspring of affected, and parents of affected children may be related. Autosomal recessive traits do skip generations.

      The rarer the trait in the general population, the more likely a consanguineous mating is involved, and the trait can appear as an isolated event in small sibships. Consaguinity is when there is a possibility of one parent being a carrier, with the recessive allele being passed through the carrier offspring and by producing an affected homozygous offspring generations later on. This is important when relating to autosomal recessive inheritance because in order for a child to be a carrier of a disease they must receive a recessive allele from both of his or her parents. The inheritance pattern of the factor VIII deficiency that is recognized in Olga's and Greg's pedigree shows that it is not an autosomal recessive trait because it is a sex-linked trait, because only guys get it. Greg's brother had it and Olga's brother had this disease. Some of the characteristics of X-linked recessive inheritance is that the disease is never passed from father to son, and this trait or disease is usually passed from an affected grandfather, through his carrier daughters, to half of his grandsons.  A son never inherits their father's defective X-chromosome, because they only inherit their father's Y-chromosome. When the mother is a carrier she will pass the affected X-chromosme to her son only one half of the time, and her daughters will not be affected because they always get a normal X-chromosome from their father.

                               

     There isn't a possibility that Greg carries the factor VIII gene, but if he did he couldn't be a carrier. There is a possibilty that Olga carriers the factor VIII gene, but it depends upon which of her mother's X chromosomes she inherited. So, her sons would be affected one half of the time but her daughters would not be affected. Olga has a one in twenty three chance of carrying the cystic fibrosis gene. Since, Greg is Asian American and within his population group the carrier frequency is 1 out of 180.  So, the possibility of producing a baby with cystic fibrosis would be 1 out of 16,560. So there chances are very slim of having a kid with that disease. The equation P+q=1 describes all of the alleles in the population. The percentage of the healthy people represents the p. The rest of the alleles must have the disease causing form which represents the q. If p is .65, then q is the other alleles that must be the disease causing form which is .35. Then, p+q=1. In all, I understand about the characteristics of autosomal dominant traits, autosomal recessive traits, and sex-linked traits.



The link to my paper about Eugenics

This is a link to my paper about Eugenics

Source: http://www.eugenicsarchive.org/eugenics/ - That is where I got my Information from.